The NeoTYPE Liver/Biliary Tumor Profile analyzes 38 biomarkers through a combination of next-generation sequencing (NGS), FISH, and IHC as listed below. Test orders include summary interpretation of all results to help guide treatment decisions. If Pan-TRK IHC is expressed or equivocal, reflex to either NTRK NGS Fusion Panel (Default) or NTRK 1-3 FISH Panel will be added. A microsatellite instability (MSI) NGS result of “indeterminate” will create a reflex to MSI by PCR as long as the tumor percentage is ≥40% and paired normal tissue is available.
NGS (32 genes + 2 biomarkers): AKT1, APC, ARID1A, ATM, BAP1, BRAF, CDKN2A, CTNNB1, EGFR, ERBB2, ERBB4, FGFR1 (mutations), FGFR2 (mutations), FGFR3 (mutations), HRAS, IDH1, IDH2, KRAS, MET, Microsatellite Instability (MSI), NF1, NOTCH1, NRAS, PBRM1, PIK3CA, PTEN, SMAD4, SMO, SRC, TERT Promoter, TP53, TSC1, TSC2, Tumor Mutation Burden (TMB)
FISH (3 FISH): FGFR2 rearrangement, MET, PTEN (tech-only available)
IHC (2 biomarkers): PD-L1 LDT, Pan-TRK (tech-only available for PD-L1)
The NeoTYPE Liver/Biliary Tumor Profile characterizes liver/biliary tumors of any histological subtype for the most significant genetic changes relevant to therapy decisions, prognosis, and clinical research. It is appropriate for patients with newly-diagnosed or recurrent disease, and for patients with resistant disease to explore options in clinical trials.
- FFPE tissue: Paraffin block preferred. Please use 10% buffered formalin fixative. Do not use zinc fixatives.
Use cold pack for transport, making sure cold pack is not in direct contact with specimen.