The NeoTYPE Discovery Profile for Hematologic Cancers analyzes 302 genes through next-generation sequencing (NGS): ABL1, ABL2, AKT1, AKT2, AKT3, ALK, ANKRD26, APC, ARAF, ARHGEF1, ARID1A, ARID1B, ARID2, ASXL1, ASXL2, ATG2B, ATM, ATP2A2, ATRX, AXL, B2M, BAP1, BCL2, BCL2L11, BCL6, BCOR, BCORL1, BCR, BIRC3, BLM, BRAF, BRCA1, BRCA2, BRINP3, BRIP1, BTK, C17orf97, CALR, CARD11, CBFB, CBL, CBLB, CBLC, CCND1, CCND2, CCND3, CD274, CD33, CD79A, CD79B, CDC25C, CDK2, CDK4, CDK6, CDKN1B, CDKN2A, CDKN2B, CEBPA, CHEK2, CIC, CIIT, CREBBP, CRLF2, CSF1R, CSF3R, CTC1, CTCF, CTNNB1, CUX1, CXCR4, CYLD, DAXX, DCK, DDX3X, DDX41, DIS3, DKC1, DNMT1, DNMT3A, EBF1, EED, EGFR, EGLN1, EGR1, ELANE, EP300, EPCAM, EPHA2, EPHA7, EPOR, ERBB2, ERBB3, ERCC4, ETNK1, ETV6, EZH2, FANCA, FANCB, FANCC, FANCD2, FANCE, FANCF, FANCG, FANCI, FANCL, FANCM, FAS, FAT1, FBXW7, FGFR1, FGFR2, FGFR3, FLT3, FOXO1, FUBP1, G6PC3, GAB2, GATA1, GATA2, GATA3, GFI1, GNA12, GNA13, GNAI2, GNAQ, GNAS, GNB1, GSKIP, H1-4, HAX1, HIF1A, HNRNPK, HRAS, ID3, IDH1, IDH2, IGF1R, IKBKB, IKZF1, IKZF3, IL7R, IRAK4, IRF4, ITPKB, JAK1, JAK2, JAK3, KDM6A, KDR, KEAP1, KIT, KLF2, KLHL6, KMT2A, KMT2C, KMT2D, KRAS, LUC7L2, MALT1, MAP2K1, MAP3K1, MAP3K14, MAPK1, MCL1, MDM2, MDM4, MED12, MEF2B, MET, MLH1, MPL, MSH2, MSH6,MTOR, MYC, MYCN, MYD88, NBN, NCAPH, NF1, NFKBIE, NHP2, NOP10, NOTCH1, NOTCH2, NOTCH3, NPM1, NRAS, NSD1, NT5C2, NTRK1, NTRK2, NTRK3, NUP214, NUP98, P2RY8, PALB2, PAX5, PDCD1LG2, PDGFRA, PDGFRB, PHF6, PIGA, PIK3CA, PIK3CD, PIK3R1, PIM1, PLCG1, PLCG2, PML, PMS2, POT1, PPM1D, PRDM1, PRPF40B, PRPF6, PRPF8, PRPS1, PTCH1, PTEN, PTPN11, PTPRC, RAC1, RAD21, RAD51C, RAD51D, RB1, RBBP6, REL, RHEB, RHOA, RICTOR, RIPK1, RIT1, RPL11, RPL35A, RPL5, RPN1, RPS10, RPS15, RPS17, RPS26, RPS7, RTEL1, RUNX1, S1PR2, SAMD9, SAMD9L, SAMHD1, SBDS,SETBP1, SETD2, SF1, SF3A1, SF3B1, SGK1, SH2B3, SLX4, SMAD4, SMARCB1, SMC1A, SMC3, SMO, SOCS1, SPEN, SRP72, SRSF2, STAG2, STAT3, STAT5B, STAT6, STK11, SUZ12, TBL1XR1, TCF3, TENT5C, TERC, TERT, TET2, TET3, THPO, TINF2, TLR2, TNFAIP3 TNFRSF14, TP53, TP63, TRAF2, TRAF3, TSC1, TSC2, U2AF1, U2AF2, UBR5, VHL, WAS, WRAP53, WT1, XPO1, ZFHX4, ZMYM3, and ZRSR2. Test reports include a summary of all results together. Note: FLT3 is performed by NGS only. FLT3 by other molecular method can be ordered as an add-on if desired.
The NeoTYPE Discovery Profile for Hematologic Cancers is a qualitative next-generation sequencing (NGS) test for the detection of DNA alterations in 302 genes of various myeloid and lymphoid disorders. The assay covers genes implicated in the pathogenesis of lymphomas and leukemias, including acute myeloid leukemia (AML), myeloproliferative neoplasms (MPN), myelodysplastic syndrome (MDS), chronic myelogenous leukemia (CML), chronic myelomonocytic leukemia (CMML), juvenile myelomonocytic leukemia (JMML), angioimmunoblastic T-cell lymphoma (AITL)/peripheral T-cell lymphoma (PTCL), chronic lymphocytic leukemia (CLL), among others. The results may provide insight into the pathobiology of the malignancy, aid in risk assessment, and identify potential therapeutic options and clinical trials based on molecular drivers.
The NeoTYPE Discovery Profile for Hematologic Cancers is intended for predictive and prognostic purposes. In some cases, it may aid in diagnosis/subtype, e.g. HCL, MDS with SF3B1, MPN, or AML.
- Bone Marrow Aspirate: 2-3 mL sodium heparin tube. EDTA tube is acceptable.
- Peripheral Blood: 3-5 mL sodium heparin tube. EDTA tube is acceptable.
- FFPE tissue: Paraffin block. Alternatively, send 1 H&E slide plus 5-10 unstained slides cut at 5 or more microns. Please use positively-charged slides and 10% NBF fixative is the recommended fixative. Do not use zinc or mercury fixatives (B5). Highly acidic or prolonged decalcification processes will not yield sufficient nucleic acid to accurately perform molecular studies.
Use refrigerated cold pack for transport. Make sure cold pack is not in direct contact with specimen.