Probes: MYCN (2p24.3) | Centromere 2
Disease(s): Brain cancer, neuroblastoma, alveolar rhabdomyosarcoma, small-cell lung cancer, prostate cancer.
The MYCN gene, which encodes the n-myc protein, is a proto-oncogene with highest expression in the developing brain and insignificant expression in normal adult tissues. Gene amplification is detected in approximately 20% of neuroblastoma and a variety of other solid tumors including 5% medulloblastoma, glioblastoma multiforme, 25% alveolar rhabdomyosarcoma, 15-20% small-cell lung cancer, 40% neuroendocrine prostate cancer, and 5% prostate adenocarcinoma. MYCN amplifications are associated with aggressive disease and/or poor outcome. Detection can be useful to stratify patients for aggressive treatment. Numerous therapies are in development.
- Bone marrow aspirate: N/A
- Peripheral blood: N/A
- Fresh, unfixed tissue: N/A
- Fluids: N/A
- Paraffin block: Send paraffin block. Also send circled H&E slide for tech-only (required).
- Cut slides: H&E slide (required) plus 4 unstained slides cut at 4-5 microns. Circle H&E slide for tech-only.
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.