Methodology
FISH
Test Description
Probes: Trisomy 8 (Cen 8) | MYC/IgH t(8;14)
Disease(s): Burkitt lymphoma, NHL
Clinical Significance
The MYC/IgH rearrangement is common in Burkitt lymphoma and diffuse large B-cell lymphoma, associated with a more aggressive tumor phenotype and a lower survival rate. MYC/IgH/CEN8 can confirm heavy chain rearrangement when MYC is rearranged.
Specimen Requirements
- Bone marrow aspirate: 1-2 mL in sodium heparin tube. EDTA tube is acceptable.
- Peripheral blood: 2-5 mL in sodium heparin tube. EDTA tube is acceptable.
- Fresh tissue: Tissue in RPMI.
- Bone Marrow/ Peripheral Blood Smear or Fresh Tissue Touch Preparation Slides: minimum 1 slide labeled with specimen type.
- Fluids: Equal parts RPMI to specimen volume.
- Fixed Cell Suspension: A client fixed cell suspension may be submitted for testing as long as it is received in 3:1 Methanol:Glacial Acetic Acid.
- Paraffin block: H&E slide (required) plus paraffin block. Circle H&E for tech-only.
- Cut slides: H&E slide (required) plus 2 unstained slides cut at 4 microns. Circle H&E for tech-only.
- Note: Please exclude biopsy needles, blades, and other foreign objects from transport tubes. These can compromise specimen viability and yield, and create hazards for employees.
Storage & Transportation
Refrigerate specimen. Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen. For fresh samples: ship same day as drawn whenever possible; specimens <72 hours old preferred.
CPT Code(s)*
88374x1 automated. Codes may differ if manual analysis is performed.
Turnaround Time
3-5 days for unfixed or FFPE specimens
Level of Service
Technical
Global
New York Approved
Yes