Methodology
Immunohistochemistry (IHC)
Test Description
Human mismatch repair protein 2 (MSH2) is involved in the initial recognition of mismatched nucleotides during the post replication mismatch repair process. Loss of MSH2 function leads to the accumulation of replication errors, which in turn may be responsible for the multiple mutations required for multistage carcinogenesis. Mutations in MSH2 gene is linked to hereditary nonpolyposis colon cancer and to sporadic cancers which exhibit microsatellite instability.
Specimen Requirements
- A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen type
or - One (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody ordered
- Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Storage & Transportation
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.
CPT Code(s)*
88342 x 1 or 88341 x 1; 88360 x 1; 88361 x 1
Turnaround Time
Global: 48 hours, Tech-Only (stain only): 24 hours
Level of Service
Global
Stain Only
New York Approved
Yes