Probes: MDM2 (12q15) | Centromere 12
This test detects amplifications of the MDM2 gene for classification of certain adipocytic tumors. MDM2 gene amplifications may appear microscopically as ring chromosomes, giant marker chromosomes, and double minutes. MDM2 amplifications are frequently detected in well-differentiated liposarcoma, which includes atypical lipomatous tumor (ALT/WDLS), and in dedifferentiated liposarcoma (DDLS). These tumors can be difficult to distinguish morphologically from other high-grade sarcomas and benign tumors. Amplifications are detected infrequently in other soft tissue sarcomas, and are not detected in benign lipomas. Drug targeting of MDM2 is an active area of clinical research.
- Bone marrow aspirate: N/A
- Peripheral blood: N/A
- Fluids: N/A
- Paraffin block: Send paraffin block. Also send circled H&E slide for tech-only (required).
- Cut slides: H&E slide (required) plus 4 unstained slides cut at 4-5 microns. Circle H&E slide for tech-only.
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.