Methodology
Immunohistochemistry (IHC)
Test Description
Ki67 is a nuclear protein that is expressed in proliferating cells. Ki67 is preferentially expressed during late G1, S, M, and G2 phases of the cell cycle, while cells in the G0 (quiescent) phase are negative for this protein. Increased proliferative activity is associated with more aggressive tumor and decreased disease-free survival period.
Note: Computer-assisted image analysis for Ki-67 is only validated for breast cancer. For neuroendocrine/carcinoid cancer, please use Ki-67 NET. For all other tumor types, please use Tech-Only (stain only) Ki-67.
Specimen Requirements
- A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen type
or - One (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody ordered
- Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Storage & Transportation
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.
CPT Code(s)*
88342 x 1 or 88341 x 1 (qualitative IHC) or 88360 (quantitative/semi-quantitative – manual) or 88361 x 1 (quantitative/semi-quantitative – computer assisted)
Turnaround Time
Global: 48 hours, Image Analysis (tech-only): 48 hours, Tech-Only (stain only): 24 hours
Level of Service
Stain Only
Global
New York Approved
Yes