Methodology
FISH
Test Description
Probes: IgH/MAFB t(14;20)
This probe combination may be ordered separately or added to any of our mye
Disease(s): Myeloma, MGUSloma FISH panels.
Clinical Significance
The translocation (14;20) is rare in multiple myeloma (MM) but associated with a poor prognosis for that diagnosis. In contrast, t(14;20) in patients with MGUS is associated with a long-term stable disease.
Specimen Requirements
- Bone Marrow Aspirate: 1-2 mL sodium heparin tube. EDTA tube is acceptable.
- Peripheral Blood: 2-5 mL sodium heparin tube. EDTA tube is acceptable.
- Fresh, Unfixed Tissue: Tissue in RPMI.
- Bone Marrow/ Peripheral Blood Smear or Fresh Tissue Touch Preparation Slides: minimum 1 slide labeled with specimen type.
- Fluids: Equal parts RPMI to specimen volume.
- Fixed Cell Suspension: A client fixed cell suspension may be submitted for testing as long as it is received in 3:1 Methanol:Glacial Acetic Acid.
- Paraffin Block or Cut Slides: Not available.
- Note: Please exclude biopsy needles, blades, and other foreign objects from transport tubes. These can compromise specimen viability and yield, and create hazards for employees.
Storage & Transportation
Use cold pack for transport, making sure cold pack is not in direct contact with specimen.
CPT Code(s)*
88374x1 automated. Codes may differ if manual analysis is performed.
Turnaround Time
3-5 days
Level of Service
Technical
Global
New York Approved
Yes