Methodology
Flow Cytometry
Test Description

Markers are CD14, CD15, CD24, CD45, CD59, CD64, CD235a (Glycophorin A), and FLAER. In validation studies, this assay was shown to detect RBC and granulocyte PNH clones with frequency down to 0.01%.

Clinical Significance

Useful for diagnosis of PNH (paroxysmal nocturnal hemoglobinuria) and monitoring response to therapy. Small PNH clones may also be identified in patients with aplastic anemia and MDS who may respond to immune modulation therapy. Also identifies patients at increased risk of developing overt PNH. This assay is consistent with International Clinical Cytometry Society (ICCS) Guidelines. NeoGenomics no longer performs PNH testing on bone marrow specimens.

Specimen Requirements
  • Peripheral blood: 1-2 mL EDTA preferred. 1-2 mL minimum in sodium heparin tube or ACD (pale yellow, no gel separator) is acceptable. Please provide a recent CBC report. Bone marrow specimens are not acceptable.
Storage and Transportation

Specimens should be received at NeoGenomics within 48 hours from collection to assure sample integrity and acceptable cell viability. Refrigerate specimen. Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

CPT Code(s)*
88184x1, 88185x7, 88187x1
Turnaround Time

1 day

Level of Service
Global
Technical

*The CPT codes provided with our test descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.