Alternative Name
FLT3, FLT3 TKD, FLT3 ITD
Methodology
Molecular
Test Description
Detection of internal tandem duplication and exon 20 tyrosine kinase domain (TKD) mutations using fragment-length analysis and PCR. Positive results identify presence of TKD mutations or report ITD results quantitatively as allelic ratio.
Clinical Significance
Testing for FLT3 and other gene mutations in AML patients with intermediate-risk cytogenetic abnormalities can improve risk stratification. The presence of an FLT3 mutation in a patient with AML implies aggressive disease.
Specimen Requirements
- Peripheral blood: 5 mL in EDTA tube.
- Bone marrow: 2 mL in EDTA tube.
- FFPE tissue: Paraffin block is preferred. Alternatively, send 1 H&E slide plus 5-10 unstained slides cut at 5 or more microns. Please use positively-charged slides and 10% NBF fixative. Do not use zinc fixatives.
Note: Test is DNA-based. Please select Extract & Hold - DNA if specimen hold service is desired.
Storage & Transportation
Use cold pack for transport, making sure cold pack is not in direct contact with specimen.
CPT Code(s)*
81245, 81246
Medicare MolDX CPT Code(s)*
81479
Turnaround Time
5 days
Level of Service
Global
New York Approved
Yes