Detection of internal tandem duplication and exon 20 tyrosine kinase domain (TKD) mutations using fragment-length analysis and bi-directional sequencing. Positive results identify specific TKD mutations or report ITD results quantitatively as allelic ratio. Testing may be performed on plasma to increase sensitivity.
This test is the NY-approved version of the FLT3 Mutation Analysis.
Testing for FLT3 and other gene mutations in AML patients with intermediate-risk cytogenetic abnormalities can improve risk stratification. The presence of an FLT3 mutation in a patient with AML implies aggressive disease.
- Peripheral blood: 5 mL in EDTA tube.
- Bone marrow: 2 mL in EDTA tube.
Use cold pack for transport, making sure cold pack is not in direct contact with specimen.