Methodology
FISH
Test Description
Probes: FGFR3/IgH t(4;14)
Disease(s): Multiple myeloma, MGUS
Clinical Significance
Available separately or as part of the Plasma Cell Myeloma FISH panels: Plasma Cell Myeloma IgH Complex FISH Panel and Plasma Cell Myeloma Prognostic FISH Panel.
To learn more about FISH testing for IgH translocations in multiple myeloma, please visit Multiple Myeloma Cytogenetic Analysis resource page.
Specimen Requirements
- Bone Marrow Aspirate: 1-2mL Sodium Heparin Tube. EDTA tube is acceptable
- Peripheral Blood: 2-5mL Sodium Heparin Tube. EDTA tube is acceptable
- Fresh, Unfixed Tissue: Tissue in RPMI
- Bone Marrow/ Peripheral Blood Smear or Fresh Tissue Touch Preparation Slides: minimum 1 slide labeled with specimen type.
- Fluids: Equal parts RPMI to specimen volume
- Fixed Cell Suspension: A client fixed cell suspension may be submitted for testing as long as it is received in 3:1 Methanol:Glacial Acetic Acid.
- Paraffin or Cut Slides: N/A
- Note: Please exclude biopsy needles, blades, and other foreign objects from transport tubes. These can compromise specimen viability and yield, and create hazards for employees.
Storage & Transportation
Refrigerate specimen. Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen. For fresh samples: ship same day as drawn whenever possible; specimens <72 hours old preferred.
CPT Code(s)*
88374x1 automated. Codes may differ if manual analysis is performed.
Turnaround Time
3-5 days
Level of Service
Technical
Global
New York Approved
Yes