Methodology
Flow Cytometry
Test Description
Available as global and tech-only. Markers are CD2, CD3, CD4, CD5, CD7, CD8, CD10, CD11b, CD11c, CD13, CD14, CD15, CD16, CD19, CD20, CD23, CD33, CD34, CD38, CD41, CD45, CD56, CD64, CD71, CD117, CD138, CD235a, FMC-7, HLA-DR, kappa, and lambda.
Clinical Significance
For diagnosis of leukemia, lymphoma, plasma cell neoplasms, and evaluation of myeloid maturation.
Specimen Requirements
- Bone Marrow Aspirate: 1-2 mL EDTA. Sodium heparin is acceptable. Lithium heparin or ACD (pale yellow/no gel separator) is not acceptable. Please provide recent CBC report.
- Peripheral Blood: 1-2 mL EDTA. Sodium heparin is acceptable. Lithium heparin or ACD (pale yellow/no gel separator) is not acceptable. Please provide recent CBC report.
- Fresh Bone Marrow Core Biopsy: 1-2cm core (length) tissue in RPMI
- Fresh/Unfixed Tissue: 0.2 cm3 minimum in RPMI
- Fluids and FNAs: Equal parts RPMI and specimen volume
- CSF: 1-2 mL recommended
- NY Clients: Please provide Date and Time of Collection.
- Note: Please exclude biopsy needles, blades, and other foreign objects from transport tubes. These can compromise specimen viability and yield, and create hazards for employees.
Storage & Transportation
Specimens should be received at NeoGenomics within 72 hours from collection to assure sample integrity and acceptable cell viability. Note: New York State samples must be received within 48 hours from collection per NYS requirements. Refrigerate specimen. Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.
CPT Code(s)*
88184(x1), 88185(x30). Add 88189(x1) for global.
Turnaround Time
24 hours
Level of Service
Technical
Global
New York Approved
Yes