Probes: EGFR (7p11.2) | Centromere 7
Disease(s): Brain, lung, colorectal, gastric, breast cancers
FISH analysis for amplifications of EGFR (aka ERBB1 or HER1) can be used for prognostic and therapeutic consideration in various solid tumors such as glioblastoma, lung, colorectal, gastric, and breast. Amplifications are typically associated with poor prognosis, while presence and level of amplification may not correspond to level of protein expression or response to anti-EGFR therapy in gastrointestinal cancers. High gene copy number may indicate poor prognosis in gastric and triple-negative breast cancers. However, its role in treating breast cancer and other tumors with EGFR/HER2/HER4 inhibitors is under investigation.
- Bone marrow aspirate: N/A
- Peripheral blood: N/A
- Fresh, unfixed tissue: N/A
- Fluids: N/A
- Paraffin block: Send paraffin block. Also send circled H&E slide for tech-only (required).
- Cut slides: H&E slide (required) plus 4 unstained slides cut at 4-5 microns. Circle H&E slide for tech-only.
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.