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Alternative Name
Epstein-Barr Virus by ISH
In Situ Hybridization (ISH)
Test Description

This probe set labels all latent EBV-infected cells, including EBV-positive lymphoblastoid cell lines and EBV infected B-cell immunoblasts in infectious mononucleosis. It also reacts with EBV-associated undifferentiated nasopharyngeal carcinomas and with Reed-Sternberg cells in almost all EBV-associated Hodgkin lymphoma cases. Global interpretation is available on head and neck specimens only; tech-only testing is available for all samples.

Clinical Significance

The Epstein-Barr virus (EBV) probe demonstrates latent EBV infection by hybridizing to abundantly expressed EBER transcripts which are concentrated in the nuclei of latently infected cells.

Specimen Requirements
  • A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen type
  • One (1) unbaked, unstained slide for H&E staining (required) and three (3) positively charged unstained slides, all cut at 4-5 microns
  • Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Storage and Transportation

Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.

CPT Code(s)*
88365 x 1 or 88364 x 1
New York Approved
Level of Service
Stain Only
Turnaround Time

Global (head and neck only): 72 hours, Tech-Only (stain only): 48 hours

*The CPT codes provided with our test descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.