Probes: DDIT3 (CHOP) (12q13)
Disease(s): Myxoid-round cell liposarcoma (M/RCLS)
The DDIT3 break-apart FISH test detects 12q13 rearrangements in myxoid-round cell liposarcoma (M/RCLS). The DDIT3 locus was formerly named CHOP. Fusion of DDIT3 and FUS genes in t(12;16)(q13;p11) is detected in >95% of cases. Occasionally observed is t(12;22)(q13;q12) in which DDIT3 and EWSR1 are fused. DDIT3 rearrangement partners will not be identified by this FISH test. For that purpose, please see the NGS Sarcoma Fusion Profile.
- Bone marrow aspirate: N/A
- Peripheral blood: N/A
- Fresh, unfixed tissue: N/A.
- Fluids: N/A
- Paraffin block: Send paraffin block. Also send circled H&E slide for tech-only (required).
- Cut slides: H&E slide (required) plus 4 unstained slides cut at 4-5 microns. Circle H&E slide for tech-only
Refrigerate specimen. Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.