Probes: CDKN2A (p16) (9p21) | Centromere 9
Disease(s): Mesothelioma, Glioma
Detection of homozygous deletions of CDKN2A (also called p16) by FISH is useful to distinguish malignant pleural mesothelioma (MPM) and diffuse malignant peritoneal mesothelioma (DMPM) from reactive mesothelial hyperplasia (RMH) and epithelial ovarian cancer (EOC). While morphologic, immunocytochemical, and immunohistochemical analyses determine the mesothelial origin of such neoplasms, CDKN2A FISH enables differentiation of benign from malignant proliferations with high specificity and positive predictive value, particularly when combined with BAP-1 IHC. Homozygous deletions have been reported in 70-80% of MPM cases and are associated with shorter survival in these patients.
- Bone marrow aspirate: N/A
- Peripheral blood: N/A
- Fresh, unfixed tissue: N/A
- Fluids: N/A
- Paraffin block: Send paraffin block. Also send circled H&E slide for tech-only (required).
- Cut slides: H&E slide (required) plus 4 unstained slides cut at 4-5 microns. Circle H&E slide for tech-only.
Use cold pack for transport, making sure cold pack is not in direct contact with specimen.