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Methodology
Flow Cytometry
Test Description

Available as global and tech-only. Available as stand-alone test (as described here) or as add-on to panels. Markers are CD3, CD19, CD45, and CD52. Additional markers may be necessary to identify a subset of abnormal cells in a background of normal cells.

Clinical Significance
Identifies CD52+ patients with B-CLL, other B-cell neoplasms, or T-cell neoplasms including mycosis fungoides (MF) and Sezary syndrome who may be considered for alemtuzumab (anti-CD52, Campath®) therapy.  See also T-Cell Therapy Panel for more extensive analysis of 10 T-cell markers. 
Specimen Requirements

  • Bone Marrow Aspirate: 1-2 mL EDTA.  Sodium heparin is acceptable.  Lithium heparin or ACD (pale yellow/no gel separator) is not acceptable.  Please provide recent CBC report.

  • Peripheral Blood: 1-2 mL EDTA.  Sodium heparin is acceptable.  Lithium heparin or ACD (pale yellow/no gel separator) is not acceptable.  Please provide recent CBC report.

  • Fresh Bone Marrow Core Biopsy: 1-2cm core (length) tissue in RPMI

  • Fresh/Unfixed Tissue: 0.2 cm3 minimum  in RPMI

  • Fluids and FNAs: Equal parts RPMI and specimen volume

  • NY Clients: Please provide Date and Time of Collection.

  • Note: Please exclude biopsy needles, blades, and other foreign objects from transport tubes. These can compromise specimen viability and yield, and create hazards for employees.

Storage and Transportation

Specimens should be received at NeoGenomics within 72 hours from collection to assure sample integrity and acceptable cell viability.  Note: New York State samples must be received within 48 hours from collection per NYS requirements. Ship same day as drawn whenever possible. Refrigerate specimen. Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

CPT Code(s)*
88184(x1), 88185(x3). Add 88187(x1) for global.
Turnaround Time

24 hours

New York Approved
Yes
Level of Service
Global
Technical

*The CPT codes provided with our test descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.