Flow Cytometry
Test Description

Available as global and tech-only. Available as stand-alone test (as described here) or as add-on to panels. Markers are CD3, CD19, CD45, and CD52. Additional markers may be necessary to identify a subset of abnormal cells in a background of normal cells.

Clinical Significance
Identifies CD52+ patients with B-CLL, other B-cell neoplasms, or T-cell neoplasms including mycosis fungoides (MF) and Sezary syndrome who may be considered for alemtuzumab (anti-CD52, Campath®) therapy.  See also T-Cell Therapy Panel for more extensive analysis of 10 T-cell markers. 
Specimen Requirements
  • BM Aspirate: 1mL EDTA: sodium heparin is acceptable; provide CBC
  • Peripheral Blood: 1mL EDTA: sodium heparin is acceptable; provide CBC
  • Fresh Bone Marrow Core Biopsy: 1-2cm core (length) tissue in RPMI
  • Fresh/Unfixed Tissue: Tissue in RPMI
  • Fluids: Equal parts RPMI and specimen volume
Storage and Transportation

Specimens should be received at NeoGenomics within 72 hours from collection to assure sample integrity and acceptable cell viability. Ship same day as drawn whenever possible. Refrigerate specimen. Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

CPT Code(s)*
88184(x1), 88185(x3). Add 88187(x1) for global.
Turnaround Time

24 hours

Level of Service

*The CPT codes provided with our test descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.