Methodology
Immunohistochemistry (IHC)
Test Description
Calponin, a calmodulin, is involved in the regulation of smooth muscle contraction. The expression of calponin is restricted to smooth muscle cells. Two isoforms of calponin exist with molecular weights of 34kDa and 29kDa. Expression of the 29kDa form is primarily restricted to muscle of the urogenital tract. Calponin also labels myoepithelial cells and can be useful in distinguishing in situ from infiltrating breast carcinoma.
Specimen Requirements
- A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen type
or - One (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody ordered
- Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Storage & Transportation
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.
CPT Code(s)*
88342 x 1 or 88341 x 1
Turnaround Time
24 hours
Level of Service
Stain Only
New York Approved
Yes