Calcitonin is secreted by thyroidal parafollicular cells of neuroectodermal origin, probably in response to hypercalcemia. The IHC demonstration of calcitonin is important: (1) For identification of early or microscopic medullary thyroid cancer (MTC), (2) To identify an MTC in the absence of amyloid deposits, (3) To distinguish non-typical forms of MTC (e.g., predominantly spindle cell or small cell patterns) from anaplastic carcinoma or malignant lymphoma, (4) To differentiate MTC with microfollicular or papillary patterns from thyroid follicular and papillary neoplasms and (5) To identify C-cell hyperplasia in association with hypercalcemia of diverse etiologies.
- A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen type
- One (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody ordered
- Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.