Methodology
Immunohistochemistry (IHC)
Test Description
The combination of CK5 + P63 + CK8/18 (Breast Triple Stain) can be useful in distinguishing ductal carcinoma in situ (DCIS) from microinvasive breast carcinoma. This multiplex can decipher between a radial scar and infiltrating carcinoma. P63 (nuclear brown) and CK5/6 (cytoplasmic brown) stain myoepithelial cells, whereas CK8/18 labels the cytoplasm (red) of all ductal or lobular epithelium.
Specimen Requirements
  • A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen type
  • or
  • One (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody ordered
  • Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Storage and Transportation
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.
CPT Code(s)*
88344 x 1
Turnaround Time
24 hours
Level of Service
Stain Only

*The CPT codes provided with our test descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.