The Brain Tumor NGS Fusion Panel is an RNA-based next-generation sequencing panel that detects gene rearrangements (fusions) with known and novel fusion partners of these 28 genes: ALK, BRAF, CIC, EGFR including EGFRvIII, EML4, ETV6, EWSR1, FGFR1, FGFR2, FGFR3, FUS, KIAA1549, MAML2, MET, MN1, MYB, MYBL1, NTRK1, NTRK2, NTRK3, PRKCA, RAF1, ROS1, STAT6, TACC3, TFG, YAP1, and ZFTA (C11orf95).
The Brain Tumor NGS Fusion Panel is intended to detect gene fusions associated with brain tumors to aid in the diagnosis, disease classification, and therapy determination as outlined in the 2021 WHO Classification of Tumors of the CNS, 5th edition. Gliomas are the most common primary brain tumors with high recurrence and mortality rates. Gene fusions are identified in 30-50% of glioblastomas (GBMs). Potentially druggable gene fusions in all GBMs include FGFR (1.2%-8.3%), EGFR (2.2%-4%), and NTRK (1.2%-1.7%).
- FFPE tissue: Paraffin block is preferred. Alternatively, send 1 H&E slide plus 5-10 unstained slides cut at 5 or more microns. Please use positively-charged slides and 10% NBF fixative. Do not use zinc fixatives.
Use cold pack for transport, making sure cold pack is not in direct contact with specimen.
- Louis DN et al. The 2021 WHO Classification of Tumors of the Central Nervous System: a summary. Neuro-Oncology. 2021;23(8):1231-1251. https://doi.org/10.1093/neuonc/noab106