The Brain Tumor NGS Fusion Panel is an RNA-based next-generation sequencing panel that detects translocations and fusions with known and novel fusion partners of these genes: ALK, BRAF, C11orf95, EGFR, EML4, ETV6, FGFR1, FGFR2, FGFR3, MET, NTRK1, NTRK2, NTRK3, ROS1, TACC3, TFG, and YAP1. EGFRvIII is also included and performed by RT-PCR.
- FFPE tissue: Paraffin block is preferred. Alternatively, send 1 H&E slide plus 5-10 unstained slides cut at 5 or more microns. Please use positively-charged slides and 10% NBF fixative. Do not use zinc fixatives.
Use cold pack for transport, making sure cold pack is not in direct contact with specimen.
- Xu, T. et al. Gene Fusion in Malignant Glioma: An Emerging Target for Next-Generation Personalized Treatment. Translational Oncology (2018) 1, 609-618.