Alternative Name
B-Cell Leukemia Translocation, B-Cell Leukemia Fusion
Methodology
Molecular
Test Description
PCR and fragment analysis for quantitative detection of IGH-BCL2 translocations associated with 70-80% of follicular lymphoma and approximately 20% of diffuse large B-cell lymphoma. Translocations involving the major (MBR), minor (MCR), and 3' MBR sub-cluster regions of BCL2 are analyzed. Positive results quantify the ratio of mutant BCL2 to internal control DNA. Testing may be performed on plasma to increase sensitivity.
Clinical Significance
Useful to confirm diagnosis of follicular lymphoma, monitor therapy effectiveness, and detect minimal residual disease or recurrence.
Specimen Requirements
- Peripheral blood: 5 mL in EDTA tube.
- Bone marrow: 2 mL in EDTA tube.
- FFPE tissue: Paraffin block is preferred. Alternatively, send 1 H&E slide plus 5-10 unstained slides cut at 5 or more microns. Please use positively-charged slides and 10% NBF fixative. Do not use zinc fixatives.
Storage and Transportation
Use cold pack for transporting blood, marrow or block, making sure cold pack is not in direct contact with specimen. Slides can be packed at room temperature.
CPT Code(s)*
81278 (as of 01/01/2021); Prior to CPT Code was 81402
Medicare MolDX CPT Code(s)*
81401
New York Approved
No
Level of Service
Global
Turnaround Time
7 days