Alternative Name
B-Cell Leukemia Translocation, B-Cell Leukemia Fusion
Methodology
Molecular
Test Description

Real-time PCR for quantitative detection of t(11;14) BCL1/IgH rearrangements. Analytical sensitivity is approximately 1 tumor cell in 1000 normal cells. Positive results are reported as a ratio between quantities of (11;14) DNA and a normal control gene. This translocation is also known as CCND1/IgH or BCL1/JH.

Clinical Significance

Testing may be used to confirm the diagnosis of mantle cell lymphoma, monitor therapy effectiveness, and detect minimal residual disease or relapse. This assay detects rearrangements involving the MTC (major translocation cluster) region. Due to breakpoint variations, this and other PCR-based assays cannot detect all BCL1/IgH translocations that are detected by FISH or cytogenetics.

Specimen Requirements
  • Peripheral blood: 5 mL in EDTA tube.
  • Bone marrow: 2 mL in EDTA tube.
  • FFPE tissue: Paraffin block is preferred. Alternatively, send 1 H&E slide plus 5-10 unstained slides cut at 5 or more microns. Please use positively-charged slides and 10% NBF fixative. Do not use zinc fixatives.
Storage and Transportation

Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

CPT Code(s)*
81401x1
Turnaround Time

7 days

Level of Service
Global

*The CPT codes provided with our test descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.