Real-time PCR for quantitative detection of t(11;14) BCL1/IgH rearrangements. Analytical sensitivity is approximately 1 tumor cell in 1000 normal cells. Positive results are reported as a ratio between quantities of (11;14) DNA and a normal control gene. This translocation is also known as CCND1/IgH or BCL1/JH.
Testing may be used to confirm the diagnosis of mantle cell lymphoma, monitor therapy effectiveness, and detect minimal residual disease or relapse. This assay detects rearrangements involving the MTC (major translocation cluster) region. Due to breakpoint variations, this and other PCR-based assays cannot detect all BCL1/IgH translocations that are detected by FISH or cytogenetics.
- Peripheral blood: 5 mL in EDTA tube.
- Bone marrow: 2 mL in EDTA tube.
- FFPE tissue: Paraffin block is preferred. Alternatively, send 1 H&E slide plus 5-10 unstained slides cut at 5 or more microns. Please use positively-charged slides and 10% NBF fixative. Do not use zinc fixatives.
Note: Test in DNA-based, suitable for Freeze & Hold option.
Use cold pack for transport, making sure cold pack is not in direct contact with specimen.
7 days