Alternative Name
Philadelphia chromosome (Ph-like) acute lymphoblastic leukemia (ALL) | Ph-like ALL
Methodology
FISH
Test Description

Probes: PDGFRb (5q32), BCR/ABL1-ASS1 t(9;22), JAK2 (9p24.1), EPOR (19p13.2) and CRLF2 (Xp22.33/Yp11.32) are included in the ALL FISH (Ph-like) Profile. Probes may be ordered separately.

Disease(s): Philadelphia chromosome (Ph-like) acute lymphoblastic leukemia (ALL), B lymphoblastic leukemia/lymphoma.

See also the ALL FUSION PROFILE.

Clinical Significance

The ALL FISH Profile (Ph-like) detects cytogenetic abnormalities commonly associated with Philadelphia chromosome (Ph-like) acute lymphoblastic leukemia (ALL). Patients with Ph-like B-ALL are associated with an unfavorable prognosis, but may potentially be amenable to inhibition with select tyrosine kinase inhibitors.

Specimen Requirements
  • Peripheral blood: 3-5 mL in sodium heparin (green-top) tube or EDTA (purple-top) tube
  • Bone marrow: 2-3 mL in sodium heparin (green-top) tube or EDTA (purple-top) tube
  • Lymphoid tissue: Transport in RPMI
  • FFPE tissue: No decalcification. Two (2) x four (4) um thick slides for each probe ordered

Note: Use refrigerated cold pack for transport. Make sure cold pack is not in direct contact with specimen. DO NOT FREEZE.

Probe Prioritization: If samples are insufficient to complete the whole FISH panel, NeoGenomics will prioritize testing in the following order: BCR/ABL1, CRLF2, JAK2, EPOR, and PDGFRβ, unless directed otherwise by the client.

Storage and Transportation

Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

CPT Code(s)*
88377x5
Turnaround Time

6 days

Level of Service
Global

*The CPT codes provided with our test descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.