How to Start Ordering from NeoGenomics Pharma Services

Download and electronically fill out one Project Submission Form and one Sample Sheet Form per project and e-mail them to pharmaservices@neogenomics.com. In addition, a printed hardcopy of each form must accompany every package that enters our facility for appropriate sample handling and processing. Incomplete paperwork may delay projects.

 

Order Forms

download Project Submission Form (PDF)
download Sample Spreadsheet Form (.xls)

 

Shipping Addresses:

Please speak with your NeoGenomics representative before shipping to ensure delivery to the appropriate location.

 

NeoGenomics - Houston
Attn: Pharma Services
7256 S. Sam Houston Pkwy W., Suite 300
Houston, TX 77085
 

NeoGenomics - Aliso Viejo
Attn: Pharma Services
31 Columbia
Aliso Viejo, CA 92656

 

Sample Submission Requirements

download Specimen requirements and handling procedures

 
For the best results, please follow these requirements:
QC Your DNA

The #1 reason for poor sequencing results is that researchers do not take the time to QC their samples, whether by OD or agarose gel. Simply performing a mini-prep is not sufficient as it does not guarantee the appropriate concentration and yield for sequencing. Please verify your sample quality and amounts before submitting.

 
DNA and Primer Sent Separately

NeoGenomics does not require premixing of DNA and primer for submissions. The main benefits of not premixing are:

  • Customers may request additional reactions to be performed on a particular DNA template without having to ship more sample
  • NeoGenomics can troubleshoot projects, such as adjusting DNA-primer concentration or choosing alternate primer sites in the event of poor sequencing quality following first round attempts
  • Template purification or amplification may be performed if necessary

For submitting DNA and primer separately, please refer to the following table:

Template Concentration Volume/RNX
Plasmid 200 ng/µL  10 µL 
PCR < 500 20-30 ng/µM  10 µL 
PCR > 500 30-50 ng/µM  10 µL 
Primer 5 µM  10 µL 
 

PLEASE NOTE: Typically we request more DNA than we need in order to have a sufficient quantity for any repeats that we may need to perform. In addition, a researcher may get different sample concentration values depending on his/her quantitation method.

 
DNA & Primer Combined

For sending combined samples, please refer to the following chart:

DNA Type Size DNA Conc. in H2O DNA Volume Total Amount  Primer Conc. Primer Vol.
Plasmid <6kb 200 ng/µl 10µl ~2 ug 5-10 µM  4 µL 
Plasmid 6-10kb 200 ng/µl 10µl ~2 ug 5-10 µM  4 µL 
Plasmid >10kb 200 ng/µl 10µl ~2 ug 5-10 µM  4 µL 
 PCR Product  <1kb 20-30 ng/µll 10µl ~300 ng 5-10 µM  4 µL 
 PCR Product 1-2kb 30-50 ng/µl 10µl ~500 ng 5-10 µM  4 µL 
 
96-Well Plate DNA Sequencing (Purified Plasmids & PCR Products)

Please submit DNA and primers at volumes/concentrations according to the chart above. Plates must be arrayed A1-H1, A2-H2...etc. as follows:

96-Well Plate

It is very important that plates are sealed and shipped FROZEN on dry ice.

Acceptable container Types Shippping Conditions Comments
96-Well Semi-Skirted PCR Plates; 384-Well PCR Plates Overnight Mail; Cooler; Dry Ice No PCR Strip Tubes
 
qPCR Sample Requirements 
Quantity Solution Concentration
2-3 ug DNA/RNA Water or TE Buffer 250 ng/ul
 
Other Common Sample Types 
Template Type Shipping Conditions
Glycerol Stocks Frozen; Dry Ice
Colonies/Agar Plates Room Temperature; Petri Dish Sized Plates Only
Libraries (Transformation & Ligation Mixtures) Frozen; Dry Ice
RNA Frozen; Dry Ice

MiSeq Sample Preparation

Fragment Library or Paired End Projects 
  • DNA must be double-stranded
  • Avoid whole genome amplification methods whenever possible
  • We recommend submitting genomic DNA at concentration of 100 ng/µL or higher
  • TruSeq fragment or paired end library: 200 – 1000 ngv
  • TruSeq custom amplicon: 500 – 1000 ng
  • Nextera fragment or paired end library: 200 – 1000 ng
  • DNA should have an OD260/280 ratio of 1.8 or more
  • Please provide a 1-2% agarose gel image of your sample with a 1 kb ladder
  • DNA should not be degraded, nor should it contain any particulate matter
     

    Next Generation Sample Preparation Guidelines

    The following guidelines are designed to give you a basic idea of how to prepare your samples prior to sending us your next generation project. By following these guidelines, you will help us produce the best possible quantity and quality of data for you. However, not every application can be covered here and each project is different so, please contact us for your free project-specific criteria.

     

    HiSeq 2500 Sample Preparation

    Fragment Library or Paired End Projects 
    • DNA must be double-stranded
    • Avoid whole genome amplification methods whenever possible
    • We recommend submitting 200 – 1000 ng of DNA at concentration of 10 ng/µL or higher
    • DNA should have an OD260/280 ratio of 1.8 or more
    • Please provide a 1-2% agarose gel image of your sample with a 1kb ladder
    • DNA should not be degraded, nor should it contain any particulate matter
    Mate-paired Library-based Projects 
    • DNA must be double-stranded
    • Avoid whole genome amplification methods whenever possible
    • For mate-paired libraries we recommend submitting 10-20 µg of DNA at concentration of 10 ng/µL
    • DNA should have an OD260/280 ratio of 1.8 or more
    • Please provide a 1-2% agarose gel image of your sample with a 1kb ladder
    • DNA should not be degraded, nor should it contain any particulate matter
    Transcriptome or small RNA-based Projects 
    • We require 2-5 µg of total RNA per sample
     

    IonTorrent Sample Preparation

    Fragment Library or Paired End Projects 
    • DNA must be double-stranded
    • Avoid whole genome amplification methods whenever possible
    • We recommend submitting genomic DNA at concentration of 100 ng/µL or higher
    • Fragment Library: 10 µg/sample (10 ng/uL in TE)
    • Amplicon Library: 50 ng/sample (10 ng/uL in TE)
    • DNA should have an OD260/280 ratio of 1.8 or more
    • Please provide a 1-2% agarose gel image of your sample with a 1kb ladder
    • DNA should not be degraded, nor should it contain any particulate matter
    Transcriptome or small RNA-based Projects 
    • We require 3-5 µg of total RNA per sample
     

    NanoString Sample Preparation Chart

    Hybridyzation-based capture Sample Preparation
    • DNA must be double-stranded
    • Avoid whole genome amplification methods whenever possible
    • We recommend submitting genomic DNA at concentration of 100 ng/µL or higher
    • Quantity: 3-5 µg is preferred
    • DNA should have an OD260/280 ratio of 1.8 or more
    • Please provide a 1-2% agarose gel image of your sample with a 1kb ladder
    • DNA should not be degraded, nor should it contain any particulate matter

    If you are unable to meet these sample requirements, please contact a NeoGeomics representative for suggestions and technical support.

    NanoString Assay Sample Type Assay Amount Required Sample Shipping
    Amount/Concentration
    Per Replicate

    nCounter XT Gene Expression

    and nCounter Elements™  

    Total RNA  > 100 ng  No less than 150 ng,
    normalized to 20 ng/µL
    Cell Lysate  10,000 cells  No less than 15,000 cells.
    Minimum 6,500 cells/µL or
    minimum 3,300 cells/µL
    for Elements
     FFPE RNA  > 100 ng no less than 150 ng,
    normalized to 20 ng/µL
    nCounter XT Single Cell
    and nCounter Elements
     Single Cells low input RNA  Varied (amplification)
    10 pg - 10 ng
    > 1 cell or 10 pg RNA
     miRNA   Total Purified RNA  > 100 ng  No less than 150 ng,
    normalized to 33 ng/µL 
    FFPE RNA > 100 ng No less than 150 ng,
    normalized to 33 ng/µL
    Plasma, Serum Biofluid 1-3 µL Purified RNA equivalent
    of > 200 g/µL
    plasma or serum
     nCounter XT Single CNV
    and nCounter Elements
    Purified Genomic DNA only  600 ng  No less than 650 ng,
    normalized to 85 ng/µL 
     nCounter XT Single ChIP
    and nCounter Elements
     Purified Genomic DNA only  Varied (5-10/µL) No less than 10/µL