How to Start Ordering from NeoGenomics Pharma Services
Download and electronically fill out one Project Submission Form and one Sample Sheet Form per project and e-mail them to pharmaservices@neogenomics.com. In addition, a printed hardcopy of each form must accompany every package that enters our facility for appropriate sample handling and processing. Incomplete paperwork may delay projects.
Order Forms
Project Submission Form (PDF) Sample Spreadsheet Form (.xls)
Shipping Addresses:
Please speak with your NeoGenomics representative before shipping to ensure delivery to the appropriate location.
NeoGenomics - Houston
Attn: Pharma Services
7256 S. Sam Houston Pkwy W., Suite 300
Houston, TX 77085
NeoGenomics - Aliso Viejo
Attn: Pharma Services
31 Columbia
Aliso Viejo, CA 92656
Sample Submission Requirements
Specimen requirements and handling procedures
For the best results, please follow these requirements:
QC Your DNA
The #1 reason for poor sequencing results is that researchers do not take the time to QC their samples, whether by OD or agarose gel. Simply performing a mini-prep is not sufficient as it does not guarantee the appropriate concentration and yield for sequencing. Please verify your sample quality and amounts before submitting.
DNA and Primer Sent Separately
NeoGenomics does not require premixing of DNA and primer for submissions. The main benefits of not premixing are:
- Customers may request additional reactions to be performed on a particular DNA template without having to ship more sample
- NeoGenomics can troubleshoot projects, such as adjusting DNA-primer concentration or choosing alternate primer sites in the event of poor sequencing quality following first round attempts
- Template purification or amplification may be performed if necessary
For submitting DNA and primer separately, please refer to the following table:
| Template | Concentration | Volume/RNX |
| Plasmid | 200 ng/µL | 10 µL |
| PCR < 500 | 20-30 ng/µM | 10 µL |
| PCR > 500 | 30-50 ng/µM | 10 µL |
| Primer | 5 µM | 10 µL |
PLEASE NOTE: Typically we request more DNA than we need in order to have a sufficient quantity for any repeats that we may need to perform. In addition, a researcher may get different sample concentration values depending on his/her quantitation method.
DNA & Primer Combined
For sending combined samples, please refer to the following chart:
| DNA Type | Size | DNA Conc. in H2O | DNA Volume | Total Amount | Primer Conc. | Primer Vol. |
| Plasmid | <6kb | 200 ng/µl | 10µl | ~2 ug | 5-10 µM | 4 µL |
| Plasmid | 6-10kb | 200 ng/µl | 10µl | ~2 ug | 5-10 µM | 4 µL |
| Plasmid | >10kb | 200 ng/µl | 10µl | ~2 ug | 5-10 µM | 4 µL |
| PCR Product | <1kb | 20-30 ng/µll | 10µl | ~300 ng | 5-10 µM | 4 µL |
| PCR Product | 1-2kb | 30-50 ng/µl | 10µl | ~500 ng | 5-10 µM | 4 µL |
96-Well Plate DNA Sequencing (Purified Plasmids & PCR Products)
Please submit DNA and primers at volumes/concentrations according to the chart above. Plates must be arrayed A1-H1, A2-H2...etc. as follows:
It is very important that plates are sealed and shipped FROZEN on dry ice.
| Acceptable container Types | Shippping Conditions | Comments |
| 96-Well Semi-Skirted PCR Plates; 384-Well PCR Plates | Overnight Mail; Cooler; Dry Ice | No PCR Strip Tubes |
qPCR Sample Requirements
| Quantity | Solution | Concentration |
| 2-3 ug DNA/RNA | Water or TE Buffer | 250 ng/ul |
Other Common Sample Types
| Template Type | Shipping Conditions |
| Glycerol Stocks | Frozen; Dry Ice |
| Colonies/Agar Plates | Room Temperature; Petri Dish Sized Plates Only |
| Libraries (Transformation & Ligation Mixtures) | Frozen; Dry Ice |
| RNA | Frozen; Dry Ice |
MiSeq Sample Preparation
Fragment Library or Paired End Projects
- DNA must be double-stranded
- Avoid whole genome amplification methods whenever possible
- We recommend submitting genomic DNA at concentration of 100 ng/µL or higher
- TruSeq fragment or paired end library: 200 – 1000 ngv
- TruSeq custom amplicon: 500 – 1000 ng
- Nextera fragment or paired end library: 200 – 1000 ng
- DNA should have an OD260/280 ratio of 1.8 or more
- Please provide a 1-2% agarose gel image of your sample with a 1 kb ladder
- DNA should not be degraded, nor should it contain any particulate matter
Next Generation Sample Preparation Guidelines
The following guidelines are designed to give you a basic idea of how to prepare your samples prior to sending us your next generation project. By following these guidelines, you will help us produce the best possible quantity and quality of data for you. However, not every application can be covered here and each project is different so, please contact us for your free project-specific criteria.
HiSeq 2500 Sample Preparation
Fragment Library or Paired End Projects
- DNA must be double-stranded
- Avoid whole genome amplification methods whenever possible
- We recommend submitting 200 – 1000 ng of DNA at concentration of 10 ng/µL or higher
- DNA should have an OD260/280 ratio of 1.8 or more
- Please provide a 1-2% agarose gel image of your sample with a 1kb ladder
- DNA should not be degraded, nor should it contain any particulate matter
Mate-paired Library-based Projects
- DNA must be double-stranded
- Avoid whole genome amplification methods whenever possible
- For mate-paired libraries we recommend submitting 10-20 µg of DNA at concentration of 10 ng/µL
- DNA should have an OD260/280 ratio of 1.8 or more
- Please provide a 1-2% agarose gel image of your sample with a 1kb ladder
- DNA should not be degraded, nor should it contain any particulate matter
Transcriptome or small RNA-based Projects
- We require 2-5 µg of total RNA per sample
IonTorrent Sample Preparation
Fragment Library or Paired End Projects
- DNA must be double-stranded
- Avoid whole genome amplification methods whenever possible
- We recommend submitting genomic DNA at concentration of 100 ng/µL or higher
- Fragment Library: 10 µg/sample (10 ng/uL in TE)
- Amplicon Library: 50 ng/sample (10 ng/uL in TE)
- DNA should have an OD260/280 ratio of 1.8 or more
- Please provide a 1-2% agarose gel image of your sample with a 1kb ladder
- DNA should not be degraded, nor should it contain any particulate matter
Transcriptome or small RNA-based Projects
- We require 3-5 µg of total RNA per sample
NanoString Sample Preparation Chart
Hybridyzation-based capture Sample Preparation
- DNA must be double-stranded
- Avoid whole genome amplification methods whenever possible
- We recommend submitting genomic DNA at concentration of 100 ng/µL or higher
- Quantity: 3-5 µg is preferred
- DNA should have an OD260/280 ratio of 1.8 or more
- Please provide a 1-2% agarose gel image of your sample with a 1kb ladder
- DNA should not be degraded, nor should it contain any particulate matter
If you are unable to meet these sample requirements, please contact a NeoGeomics representative for suggestions and technical support.
| NanoString Assay | Sample Type | Assay Amount | Required Sample Shipping Amount/Concentration Per Replicate |
|
nCounter XT Gene Expression and nCounter Elements™ |
Total RNA | > 100 ng | No less than 150 ng, normalized to 20 ng/µL |
| Cell Lysate | 10,000 cells | No less than 15,000 cells. Minimum 6,500 cells/µL or minimum 3,300 cells/µL for Elements |
|
| FFPE RNA | > 100 ng | no less than 150 ng, normalized to 20 ng/µL |
|
| nCounter XT Single Cell and nCounter Elements |
Single Cells low input RNA | Varied (amplification) 10 pg - 10 ng |
> 1 cell or 10 pg RNA |
| miRNA | Total Purified RNA | > 100 ng | No less than 150 ng, normalized to 33 ng/µL |
| FFPE RNA | > 100 ng | No less than 150 ng, normalized to 33 ng/µL |
|
| Plasma, Serum Biofluid | 1-3 µL | Purified RNA equivalent of > 200 g/µL plasma or serum |
|
| nCounter XT Single CNV and nCounter Elements |
Purified Genomic DNA only | 600 ng | No less than 650 ng, normalized to 85 ng/µL |
| nCounter XT Single ChIP and nCounter Elements |
Purified Genomic DNA only | Varied (5-10/µL) | No less than 10/µL |