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Ordering Logistics

How to Start Ordering from NeoGenomics Pharma Services

Download and electronically fill out one Project Submission Form and one Sample Sheet Form per project and e-mail them to seq.sales@neogenomics.com. In addition, a printed hardcopy of each form must accompany every package that enters our facility for appropriate sample handling and processing. Incomplete paperwork may delay projects.

Order Forms

download Project Submission Form
download Sample Sheet Form

Shipping Addresses:

(Speak with your NeoGenomics representative before shipping to ensure delivery to the appropriate location)

NeoGenomics - Houston
(Also known as Seqwright Genomic Services)
Attn: New Order Request
2575 West Bellfort
Suite 2001
Houston, TX 77054

NeoGenomics - Aliso Viejo
Attn: Pharma Services
31 Columbia
Aliso Viejo, CA 92656

Sample Submission Requirements

download Specimen requirements and handling procedures

For the best results, please follow these requirements:
QC Your DNA

The #1 reason for poor sequencing results is that researchers do not take the time to QC their samples, whether by OD or agarose gel. Simply performing a mini-prep is not sufficient as it does not guarantee the appropriate concentration and yield for sequencing. Please verify your sample quality and amounts before submitting.

DNA and Primer Sent Separately

NeoGenomics does not require premixing of DNA and primer for submissions. The main benefits of not premixing are:

  • Customers may request additional reactions to be performed on a particular DNA template without having to ship more sample
  • NeoGenomics can troubleshoot projects, such as adjusting DNA-primer concentration or choosing alternate primer sites in the event of poor sequencing quality following first round attempts
  • Template purification or amplification may be performed if necessary

For submitting DNA and primer separately, please refer to the following table:

 Template Concentration  Volume / RXN 
Plasmid  200 ng/µL  10µL 
PCR < 500  20-30 ng/µL  10µL 
PCR > 500  30-50 ng/µL 10µL 
Primer  5µM  10µL 

PLEASE NOTE: Typically we request more DNA than we need in order to have a sufficient quantity for any repeats that we may need to perform. In addition, a researcher may get different sample concentration values depending on his/her quantitation method.

DNA & Primer Combined

For sending combined samples, please refer to the following chart:

DNA Type Size DNA Conc. in H2O DNA Volume Total Amount  Primer Conc. Primer Vol. 
 Plasmid  <6kb  200 ng/µl  10µl  ~2 ug 5-10 µM  4 µl 
 Plasmid  6-10kb  200 ng/µl  10µl  ~2 ug  5-10 µM  4 µl
 Plasmid  >10kb  200 ng/µl  10µl  ~2 ug  5-10 µM  4 µl
 PCR Product  <1kb 20-30 ng/µll  10µl  ~300 ng  5-10 µM  4 µl
 PCR Product  1-2kb  30-50 ng/µl  10µl  ~500 ng  5-10 µM  4 µl

 

96-Well Plate DNA Sequencing (Purified Plasmids & PCR Products)

Please submit DNA and primers at volumes/concentrations according to the chart above. Plates must be arrayed A1-H1, A2-H2...etc. as follows:

96-Well Plate DNA Sequencing

It is very important that plates are sealed and shipped FROZEN on dry ice

Acceptable Container Types Shipping Conditions Comments
 96-Well Semi-Skirted PCR Plates; 384-Well PCR Plates Overnight Mail; Cooler; Dry Ice No PCR Strip Tubes

 

 

qPCR Sample Requirements 
Quantity Solution Concentration
 2-3 ug DNA/RNA Water or TE Buffer 250 ng/ul

 

Other Common Sample Types 
Template Type Shipping Conditions
Glycerol Stocks Frozen; Dry Ice
Colonies/Agar Plates Room Temperature; Petri Dish Sized Plates Only
Libraries (Transformation & Ligation Mixtures) Frozen; Dry Ice
RNA Frozen; Dry Ice

 

MiSeq Sample Preparation

Fragment Library or Paired End Projects 
  • DNA must be double-stranded
  • Avoid whole genome amplification methods whenever possible
  • We recommend submitting Genomic DNA at concentration of 100 ng/µL or higher
  • TruSeq Fragment or Paired End Library: 200 – 1000 ngv
  • TruSeq Custom Amplicon: 500 – 1000 ng
  • Nextera Fragment or Paired End Library: 200 – 1000 ng
  • DNA should have an OD260/280 ratio of 1.8 or more
  • Please provide a 1-2% agarose gel image of your sample with a 1 kb ladder
  • DNA should not be degraded, nor should it contain any particulate matter

 

Next Generation Sample Preparation Guidelines

The following guidelines are designed to give you a basic idea of how to prepare your samples prior to sending us your next generation project. By following these guidelines, you will help us produce the best possible quantity and quality of data for you. However, not every application can be covered here and each project is different so, please contact us for your free project-specific criteria.

HiSeq 2500 Sample Preparation

Fragment Library or Paired End Projects 
  • DNA must be double-stranded
  • Avoid whole genome amplification methods whenever possible
  • We recommend submitting 200 – 1000 ng of DNA at concentration of 10 ng/µL or higher
  • DNA should have an OD260/280 ratio of 1.8 or more
  • Please provide a 1-2% agarose gel image of your sample with a 1kb ladder
  • DNA should not be degraded, nor should it contain any particulate matter
Mate-paired Library-based Projects 
  • DNA must be double-stranded
  • Avoid whole genome amplification methods whenever possible
  • For mate-paired libraries we recommend submitting 10-20 µg of DNA at concentration of 10 ng/µL
  • DNA should have an OD260/280 ratio of 1.8 or more
  • Please provide a 1-2% agarose gel image of your sample with a 1kb ladder
  • DNA should not be degraded, nor should it contain any particulate matter
Transcriptome or small RNA-based Projects 
  • We require 2-5 µg of total RNA per sample

 

IonTorrent Sample Preparation

Fragment Library or Paired End Projects 
  • DNA must be double-stranded
  • Avoid whole genome amplification methods whenever possible
  • We recommend submitting Genomic DNA at concentration of 100 ng/µL or higher
  • Fragment Library: 10 µg/sample (10 ng/uL in TE)
  • Amplicon Library: 50 ng/sample (10 ng/uL in TE)
  • DNA should have an OD260/280 ratio of 1.8 or more
  • Please provide a 1-2% agarose gel image of your sample with a 1kb ladder
  • DNA should not be degraded, nor should it contain any particulate matter
Transcriptome or small RNA-based Projects 
  • We require 3-5 µg of total RNA per sample

NanoString Sample Preparation Chart

Hybridyzation-based capture Sample Preparation

  • DNA must be double-stranded
  • Avoid whole genome amplification methods whenever possible
  • We recommend submitting Genomic DNA at concentration of 100 ng/µL or higher
  • Quantity: 3-5 µg is preferred
  • DNA should have an OD260/280 ratio of 1.8 or more
  • Please provide a 1-2% agarose gel image of your sample with a 1kb ladder
  • DNA should not be degraded, nor should it contain any particulate matter

If you are unable to meet these sample requirements, please contact a NeoGeomics representative for suggestions and technical support.

 
nanoString Assay Sample type Assay Amount Required Sample Shipping
Amount/concentration Per Replicate
nCounter XT Gene Expression
and nCounter ElementsTM		 Total RNA > 100ng no less than 150ng, normalized to 20ng/µl
Cell Lysate 10,000 cells no less than 15,000 cells. Minimum 6,500 cells/µl
or minimum 3,300 cells/µl for Elements
FFPE RNA > 100ng no less than 150ng, normalized to 20ng/µl
nCounter XT Single Cell
and nCounter Elements		 Single Cells low input RNA varied (amplification)
10pg - 10ng		 > 1cell or 10pg RNA
miRNA Total Purified RNA > 100ng no less than 150ng, normalized to 33ng/µl
FFPE RNA > 100ng no less than 150ng, normalized to 33ng/µl
Plasma, Serum Biofluid 1-3 µl purified RNA equivalent of > 200g/µl
plasma or serum
nCounter XT Single CNV
and nCounter Elements		 Purified Genomic DNA only 600ng no less than 650ng, normalized to 85 ng/µl
nCounter XT Single ChIP
and nCounter Elements		 Purified Genomic DNA only varied (5-10/µl ) no less than 10/µl