Vectra® Polaris™

High Throughput Multiplexing Methodology

Vectra® Polaris: High Throughput Multiplexing Methodology

NeoGenomics offers an end-to-end Vectra Polaris service solution, using an optimized and high-throughput workflow beginning with custom assay development through multiplex imaging and analysis of 2-6 markers on a single FFPE slide. The Vectra Polaris is a fully-enclosed instrument with touchless slide automation that allows for a streamlined staining, imaging and analysis workflow ideal for implementing refined low-order multiplex panel sets with the potential for real-time testing applications.

Vectra Polaris

Vectra Polaris multiplexing services by NeoGenomics include:

  • Automated staining workflow with Opal TSA-based amplification kit 
  • Whole slide multispectral imaging at high throughput with up to 6 markers with continuous slides loading (80+ slides)
  • Expert medical, scientific, and bioinformatics consultation within a single organization
  • Custom assay design and verification
  • Assay transfer and validation 
  • Unambiguous immune cell co-expression and co-localization
  • Spatial analytics 
  • Integrated with RNAScope 

Vectra Polaris Image Analysis:

  • Phenoptics & inForm®: spectrally unmixing images, AFR and pathology views
  • HALO® or Visiopharm®: tissue seg, cell seg, phenotyping, scoring and spatial distribution analysis
     
Validated Antibody Panels or Custom Panels
We have commonly used antibody panels pre-validated and ready for use immediately (see list below). We can also develop custom panel(s) specific for your study.
Immune Cell Panel #1

CD3
CD4
CD8
FoxP3
CD68
PanCK

Immune Cell Panel #2

CD3
CD163
HLA-DR
CD68
CD56
CD20

Immune Cell Panel #3

CD8
FoxP3
PD-1
CD68
PD-L1
PanCK/SOX10

Immune Cell Panel #4

CD19
CD68
CD56
HLA-DR
PD-L1
PanCK

T cell Panel #1

CD3
CD4
CD8
CD45RO
FoxP3
PanCK

T cell Panel #2

CD4
CD8
PD-1
LAG3
TIGIT

T cell Panel #3

CD3
CD4
CD8
FoxP3
PD-1
PanCK

T cell Panel #4

CD3
CD4
CD8
FoxP3
PanCK

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