Combined Protein IF and RNA ISH assay on the same FFPE slide
MultiOmyx technology is not limited to only interrogating protein biomarkers. It can be combined with additional “omics” such as RNA ISH RNAScope assay. Utilizing advanced biochemistry workflow, imaging, and image registration, protein IF expression data can be precisely overlaid with RNA ISH dots.
Direct correlation between protein expression to mRNA expression at single cell resolution
Utilizing a single FFPE slide, RNAScope assay was performed first, followed by MultiOmyx IF staining. Top row shows CRC sample stained for CD3 and NakATPase and probed with RNAScope dapB negative control probe. Bottom row shows the same CRC sample stained for CD3 and NakATPase and probed with RNAScope PPIB positive control probe.