Sanger Sequencing

The Right Partner for Your Sanger Sequencing Project

With more than 20 years of Sanger sequencing experience, including a bank of AB 3730xl instruments, NeoGenomics Laboratories is an industry leader and reliable partner for sequencing services.

Sample Type/Minimum Requirements

• FFPE, ≥ 10 unstained slides
• Cell culture, 10⁶ cells
• Virus
• Plasmid, BAC (500ng/reaction), 150 ng/µL

Regulatory Sequencing

• CLIA-Certified and CAP-accredited  facility
• FDA submission quality reports
• Dedicated Quality Assurance supporting personnel
• TAT: 1-2 weeks

SNP Sequencing

• Assay development and validation with many pre-validated genes already available
• Mutation profile and sequencing report available
• Experienced with tumor sample and working with parrafin-embedded tissues
• Bi-directional sequencing, quality scores, and quality control system to ensure true SNP discovery (limited of detection to 20% mutation frequency)
• Secondary validations through allelic discrimination qPCR services
• TAT: 1-2 weeks

Primer Walking for Unknown Plasmid Inserts

• Design and synthesize custom primers as needed, followed by a round of sequencing.
• This process is then repeated until your region of interest is completely sequenced and assembled.
• Single-strand  and double-strand  primer walking
• TAT: 1-2 weeks

High Throughput Sequencing

• >650bp Q20 read lengths typical
• >90% average pass rate
• 96 - well format
• TAT: 24-48 hours