The Right Partner for Your Sanger Sequencing Project
With more than 20 years of Sanger sequencing experience, including a bank of AB 3730xl instruments, NeoGenomics Laboratories is an industry leader and reliable partner for sequencing services.
Sample Type/Minimum Requirements
- FFPE, ≥ 10 unstained slides
- Cell culture, 106 cells
- Virus
- Plasmid, BAC (500ng/reaction), 150 ng/µL
Regulatory Sequencing
- CLIA-Certified and CAP-accredited facility
- FDA submission quality reports
- Dedicated Quality Assurance supporting personnel
- TAT: 1-2 weeks
SNP Sequencing
- Assay development and validation with many pre-validated genes already available
- Mutation profile and sequencing report available
- Experienced with tumor sample and working with parrafin-embedded tissues
- Bi-directional sequencing, quality scores, and quality control system to ensure true SNP discovery (limited of detection to 20% mutation frequency)
- Secondary validations through allelic discrimination qPCR services
- TAT: 1-2 weeks
Primer Walking for Unknown Plasmid Inserts
- Design and synthesize custom primers as needed, followed by a round of sequencing.
- This process is then repeated until your region of interest is completely sequenced and assembled.
- Single-strand and double-strand primer walking
- TAT: 1-2 weeks
High Throughput Sequencing
- >650bp Q20 read lengths typical
- >90% average pass rate
- 96 - well format
- TAT: 24-48 hours