Enhance your Targeted Sequencing Projects

NeoGenomics provides targeted sequencing services and genomic enrichment using Agilent SureSelect™, HaloPlex™, and Illumina TruSeq™, the leading target capture technologies in the sequencing industry. Customers can enhance the throughput of their exome sequencing pro

 

Agilent Technologies - HaloPlex

NeoGenomics offers targeted sequencing using the HaloPlex™ capture system from Agilent. Select up to 5Mb of target regions including whole genes or exons for deep sequencing and highly sensitive variant detection. Your can multiplex up to 96 samples simultaneously on one sequencing run, and we will deliver your data on your terms, including customized data reporting through our cutting-edge bioinformatics pipeline.

 

Agilent Technologies - SureSelect

As a Certified Service Provider of the SureSelect Target Enrichment System, SeqWright Genomic Services has obtained the ability to improve the cost- and process-efficiency of next-generation sequencing. Based on an extremely efficient hybrid selection technique, SureSelect can be automated and easily scaled to meet the needs of larger sequencing projects. This is unlike any other commercially available target enrichment method. With sample input requirements at or below 3 µg of gDNA, researchers can perform highly targeted next-generation sequencing on the most precious of samples.

 

Next Generation Platforms Compatible With Capture Array Target Enrichment:

  • Illumina HiSeq™ 2500 (Official Service Provider)
  • Illumina MiSeq™
  • Ion Torrent PGM™
  • Ion Torrent Proton

Each of the above systems has strengths which lend themselves to certain types of projects. Please contact one of our representatives and provide them with the details of your project so that we may recommend a solution which will achieve your goals in the most efficient manner possible.

 

Applications

Efficiency & Coverage Depth: Leverage the full throughput of the sequencing platform by specifically targeting only the genes, exons or regions of interest.

The applications of sequence capture array, targeted enrichment and microfluidic technology combined with next generation genomics are as broad as they are powerful. They enable the average researcher to take on projects from multifactorial diseases studies to the analysis of multiple gene sets involved in specific metabolic pathways. General applications of these technologies include:

  • Resequencing of Exonic Regions
  • Polygenic Disease Studies
  • Genetic Marker Development
  • Large Genomic Loci Resequencing
  • Resequencing of Candidate Gene Sets
  • Metabolic Pathway Studies
 

Bioinformatics and Deliverables:

Identifying sequence variants (SNPs and InDels) is very important for clinicians to make decisions regarding the diagnosis of patients with genetic disease. NeoGenomics can use a variety of open source software such as SAMTOOLS and snpEff to identify and annotate variants in captured DNA sequences. As a certified service provider for the Agilent SureSelect Target Enrichment System for Human Exome Capture as well as Custom Target Capture Arrays, we can provide our customers with industry-leading DNA capture technologies as well as comprehensive bioinformatics support. With all of these tools in hand, our customers can focus on using their results to identify variations related to disease-causing protein structural and functional changes. The following files will be provided with your Captured DNA-Seq project:

  • Sorted-NoDup.bam: This file contains information about sequence alignment
  • Sorted-NoDup.bam.bai: This is the index file for sorted.bam. Users can load both the sorted.bam and sorted.bam.bai files into software such as Integrative Genomic View to view the variants detected
  • Var.vcf: This file contains all the variants and their annotations. The file is in standard Variant Call Format (.vcf). Users can open this file with WordPad

 More info on Bioinformatics and Data Analysis