RNA Sequencing Highlights
Comprehensive Genomic Information – Gene expression, SNPs, deletions, splice variations, novel transcripts and rearrangements, non-coding and miRNAs
No Probe Limitations – Sequencing accomplished without a reference genome, and identification of unknown exons at single-nucleotide resolution
Large Dynamic Range – Fine-tune RNA-Seq data output based on project requirements
Data Analysis and Visualization Tools – Comprehensive bioinformatics for project-specific requirements
Sample Type/Minimum Requirements:
- FFPE, ≥ 10 unstained slides
- Cell culture, 106 cells
- Frozen section, >1 mg
- Total RNA, 100 ng – 1 µg
- TAT: 2-3 weeks, additional 1-2 weeks for analysis
Sample Prep and rRNA Depletion:
- Illumina
- Life Technologies
- Single-cell RNA-Seq with Clontech™ cDNA synthesis kits
Deliverables:
- Differential expression analysis, splicing and promoter use
- Matched tumor and normal or pre- and post-drug treatment (two-way or multi-variable comparison)
- Isoform characterization
- Identification of SNPs/variants
- Pathway analysis
QC and Bioinformatics:
- Basic statistics data - read length, data output, GC content, read counts
- Quality scores
