Methodology
FISH
Test Description
Probes: CCND1/IgH t(11;14)
Disease(s): Mantle cell lymphoma, NHL, multiple myeloma
Clinical Significance
Available separately or as part of the NHL FISH Panel or Plasma Cell Myeloma IgH Complex FISH Panel
To learn more about FISH testing for IgH translocations in multiple myeloma, please visit Multiple Myeloma Cytogenetic Analysis resource page.
Specimen Requirements
- Bone Marrow Aspirate: 1-2mL Sodium Heparin Tube. EDTA tube is acceptable
- Peripheral Blood: 2-5mL Sodium Heparin Tube. EDTA tube is acceptable
- Fresh, Unfixed Tissue: Tissue in RPMI
- Bone Marrow/ Peripheral Blood Smear or Fresh Tissue Touch Preparation Slides: minimum 1 slide labeled with specimen type.
- Fluids: Equal parts RPMI to specimen volume
- Fixed Cell Suspension: A client fixed cell suspension may be submitted for testing as long as it is received in 3:1 Methanol:Glacial Acetic Acid.
- Paraffin Block: H&E slide (required) plus paraffin block. Circle H&E for tech-only.
- Cut Slides: H&E slide (required) plus 2 unstained slides cut at 4 microns. Circle H&E for tech-only.
- Note: Please exclude biopsy needles, blades, and other foreign objects from transport tubes. These can compromise specimen viability and yield, and create hazards for employees.
Storage & Transportation
Refrigerate specimen. Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.
CPT Code(s)*
88374x1automated. Codes may differ if manual analysis is performed.
Turnaround Time
3-5 days for both unfixed and FFPE specimens
Level of Service
Technical
Global
New York Approved
Yes