FISH (Fluorescence in situ hybridization)


A comprehensive set of panels and probes for hematologic disease and solid tumors 

Fluorescence in situ hybridization (FISH) can help identify subtle or sub-microscopic structural rearrangements, variant chromosomes, and low-frequency abnormalities not readily detectable by classic cytogenetics. FISH incorporates three technologies – cytogenetics, DNA hybridization, and fluorescent microscopy – to provide a uniquely informative combination of resolution and breadth. FISH is also called Molecular Cytogenetics.

NeoGenomics has provided high-quality FISH testing for over a decade.  We provide our clients a full menu of panels and probes with flexible ordering options to enable rapid and cost-effective characterization of solid tumors, leukemias, lymphomas, and myeloid disorders. We pioneered technical-only FISH testing in the mid-2000s and have since completed well over 100,000 tech-only cases with our clients.  

Highlights of our FISH program

  • Breadth of HemeFISH™ hematologic probe library is known throughout the industry
  • Comprehensive solid tumor menu, including HER2 Equivocal testing for breast cases, enables timely clinical decision-making
  • Tech-Only FISH options available for >95% of probes to keep care local and help control cost through highly individualized probe selection
  • Live webinars and on-demand training library NeoUniversity™ enhance global and tech-only clients’ technical and clinical understanding of results
  • FISH enhances multiplex tumor profiling in our NeoTYPE™ Cancer Profiles (with global and tech-only FISH options)
  • Expanded AML, MDS, and Plasma Cell Myeloma panels align with newest guidelines to increase confidence in patient diagnosis, prognosis, and treatment (SWOG/ECOG, IPSS-R, and IMWG respectively)
  • Plasma Cell Enrichment is performed upon request and improves ability to diagnose MM/MGUS patients

Specimen and ordering information

Specimen requirements

Acceptable specimen types vary by test, so please check details for the test of interest. NeoGenomics’ FISH testing is routinely performed on the following specimens:

  • Bone marrow aspirate: 1-2 mL sodium heparin tube. EDTA tube is acceptable
  • Peripheral blood: 2-5 mL sodium heparin tube. EDTA tube is acceptable
  • Paraffin block: FFPE block (add circled H&E slide for tech-only)
  • Cut slides: Unstained slides cut at 4-5 microns plus H&E slide (circle H&E slide for tech-only)

Ordering options

FISH tests can be ordered as disease-specific panels, and probe sets within panels may be ordered individually for detection of single abnormalities. Probes are available on a global and tech-only basis, with the very few global-only exceptions noted in the detailed online description of that test.

Learn more

See our full list of FISH tests with printable descriptions and specimen requirements.
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